About Gene Design

Gene Design is a web tool for designing oligonucleotides for PCR-based gene synthesis, powered by DNAWorks — originally developed by David Hoover at the NIH.

How It Works

1. Enter your protein or DNA sequence
2. Set your desired oligonucleotide parameters (length, melting temperature)
3. DNAWorks optimizes codon usage and designs overlapping oligonucleotides
4. Download the results and order your oligos

Features

• Codon optimization for multiple organisms
• Melting temperature uniformity across overlaps
• Repeat and mispriming detection
• Restriction site avoidance
• Multiple solution generation

Reference

Hoover, D.M. and Lubkowski, J. (2002) DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis. Nucleic Acids Research, 30(10):e43.